醫(yī)學(xué)論文范文:RNA干擾抑制Survivin表達(dá)對人喉癌細(xì)胞藥物敏感性的影響

醫(yī)學(xué)論文范文:RNA干擾抑制Survivin表達(dá)對人喉癌細(xì)胞藥物敏感性的影響

【摘要】 目的：探討RNA干擾技術(shù)抑制Survivin基因表達(dá)對增強(qiáng)人喉癌HEP��2細(xì)胞化療藥物敏感性。方法:針對Survivin mRNA序列設(shè)計(jì)合成小干擾RNA(siRNA)的DNA模板,構(gòu)建pRNAT�睸urvivin重組表達(dá)載體，轉(zhuǎn)染人喉癌HEP��2細(xì)胞;通過RT�睵CR和Western blot分析其對HEP��2細(xì)胞內(nèi)源性Survivin表達(dá)的影響;四甲基偶氮唑藍(lán)(MTT)法觀察HEP��2細(xì)胞對化療藥物5�睩U和優(yōu)福定敏感性的改變。結(jié)果:成功構(gòu)建pRNAT�睸urvivin重組質(zhì)粒,并成功轉(zhuǎn)染HEP��2細(xì)胞;RT�睵CR和Western blot結(jié)果證實(shí)重組質(zhì)粒在mRNA及蛋白水平分別顯著抑制Survivin基因表達(dá)(P<0.01);MTT及流式細(xì)胞儀結(jié)果證明在5�睩U和優(yōu)福定聯(lián)合作用下，pRNAT�睸urvivin組HEP��2細(xì)胞的生長抑制率明顯增高(P<0.01)。結(jié)論:pRNAT�睸urvivin可抑制Survivin在人喉癌HEP��2細(xì)胞中的表達(dá),并增強(qiáng)HEP��2細(xì)胞對化療藥物敏感性。

【關(guān)鍵詞】 Survivin RNA干擾 喉癌 藥物敏感性

Effect of Inhibited Expression of Survivin by RNA Interference on the Drug Sensitivity in Human Laryngocarcinoma Cells CHEN Xiang�瞛un,YU Zi�瞶hong,LI Guo�瞴i^ (Department of Otolaryngology,Taihe Hospital,Yunyang Medical College,Shiyan,Hubei 442000,China)

Abstract:Objective To explore the effect of inhibited expression of survivin by RNA interference on sensitivity of HEP��2 cells to chemotherapeutic drugs. Methods One pair of DNA template coding siRNA was designed and synthesized against Survivin mRNA sequence,the pRNAT�睸urvivin recombinant expression vector was constructed and transfected into HEP��2 cells.The effect of endogenous survivin expression in HEP��2 cells by RNA interference was analyzed with RT�睵CR and Western blot.The changes of sensitivity to 5�睩U and UFT in HEP��2 cells were observed with MTT.Results Recombinant plasmid pRNAT�睸urvivin was constructed and transfected into HEP��2 cells successfully,recombinant plasmid pRNAT�睸urvivin could significantly inhibit the expression of Survivin in HEP��2 cells (P<0.01),which was demonstrated by RT�睵CR and Western blot.The growth inhibitory rate of HEP��2 cells in pRNAT�睸urvivin group was significantly increased under the combined effect of 5�睩U and UFT(P<0.01),which was demonstrated by MTT and flow cytometry. Conclusion pRNAT�� Survivin could inhibit the expression of Survivin,enhance the sensitivity of HEP��2 cells to chemotherapeutic drugs醫(yī).學(xué)全.在.線網(wǎng)站f1411.cn.

Key words: Survivin;RNA interference;Laryngocarcinoma;Sensitivity

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