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醫(yī)學免費論文:骨髓基質細胞體外三維培養(yǎng)的研究
【摘要】 目的：建立骨髓基質細胞(BMSC)體外三維培養(yǎng)的方法，并觀察其細胞生長形態(tài)及生物學特征。方法：制備鼠尾膠原，對BMSC進行三維培養(yǎng)，共聚焦顯微鏡觀察細胞形態(tài)，MTT、ALP鈣鈷、蘇丹Ⅲ染色觀察BMSC增殖及分化。結果：三維培養(yǎng)的BMSC早期呈現(xiàn)集落生長特點，后逐漸向不同的形態(tài)轉變，與二維培養(yǎng)相比，細胞增殖峰值提前，且向成骨及脂肪細胞分化能力增強。結論：BMSC在體外三維培養(yǎng)模式下生長良好，能夠繼續(xù)保持其自我更新能力及分化潛能。
【關鍵詞】 骨髓基質細胞;三維培養(yǎng)
Three-dimensional Cultivation of Bone Marrow Stromal Cells in vitroWU Yan，YANG Jian，ZHANG Hong-kao，LIU Ji-jun,LI Dong-sheng (Department of Cardiology;Department of Orthopedics，Renmin Hospital,Yunyang Medical College,Shiyan,Hubei 442000,China)Abstract:Objective To establish a method for three-dimensional cultivation of bone marrow stromal cells(BMSCs) in vitro, and to observe its growth morphology and biological characteristics.Methods BMSC were cultured with prepared collagen in a manner of three-dimensional cultivation.The morphology of BMSCs was observed under confocal microscopy,the proliferation and differentiation of BMSCs were detected with MTT,Ca-co staining and sudan-Ⅲ staining.Results BMSCs grew as colony-like at early stage,and then differentiated to different forms.Compared with two-dimensional cultivation,the peak of proliferation was in advance,and the capacity of differentiation to osteoblast and adipocyte was strengthened.Conclusion Three-dimensional cultivation of BMSCs could maintain their capacity of self-renewal and differentiation potential,and cells could grow very well醫(yī).學.全.在.線f1411.cn.
Key words:Bone marrow stromal cell;Three-dimensional cultivation
骨髓基質細胞因其在二維培養(yǎng)中具有較強的自我更新能力及多分化潛能，而被認為是基因治療中合適的種子細胞。但是細胞二維培養(yǎng)與體內環(huán)境有較大差別，沒有了基質的支持，細胞貼壁生長，可能會失去原有的形態(tài)特征及生長分化能力。三維培養(yǎng)系統(tǒng)為細胞提供了立體的生長空間，能更好的模擬細胞在體內的生理狀態(tài)。本實驗在體外模擬體內環(huán)境,構建BMSCs三維培養(yǎng)體系，使細胞所處的環(huán)境盡可能接近體內,進一步研究BMSCs的生物學性能。
