大黃素對(duì)模擬冷缺血再灌注后肝細(xì)胞內(nèi)鈣離子濃度及細(xì)胞凋亡的影響

醫(yī)學(xué)論文范文:大黃素對(duì)模擬冷缺血再灌注后肝細(xì)胞內(nèi)鈣離子濃度及細(xì)胞凋亡的影響

【摘要】 目的 研究中藥大黃素對(duì)模擬冷缺血再灌注后肝細(xì)胞內(nèi)鈣離子濃度及細(xì)胞凋亡的影響。方法 體外培養(yǎng)肝細(xì)胞株HL��7702，隨機(jī)分為對(duì)照組和大黃素處理組。對(duì)照組未予大黃素處理，大黃素處理組按100、10、1μmol/L分為高、中、低3個(gè)濃度組，建立模擬冷缺血再灌注模型，流式細(xì)胞技術(shù)檢測(cè)各組細(xì)胞內(nèi)鈣離子濃度及細(xì)胞凋亡水平，分別檢測(cè)各組細(xì)胞培養(yǎng)上清液乳酸脫氫酶水平。結(jié)果 冷缺血8h再灌注6h后，高、中、低濃度大黃素處理組鈣離子熒光強(qiáng)度分別為(24.12±0.51)、(26.35±1.34)、(39.12±1.94)，均顯著低于對(duì)照組的105.29±1.01(P<0.01)。高、中、低濃度大黃素處理組細(xì)胞凋亡率分別為(5.46±0.41)%、(10.64±0.64)%、(11.90±0.50)%，均顯著低于對(duì)照組的(25.40±1.41)%(P<0.01)。高、中濃度大黃素處理組上清液LDH含量分別為(179.67±18.57)u/L、(198.83±14.22)u/L，顯著低于對(duì)照組的(351.33±34.16)u/L(P<0.01)。結(jié)論 大黃素可降低模擬冷缺血再灌注后的肝細(xì)胞內(nèi)鈣離子濃度，抑制細(xì)胞凋亡，減輕肝細(xì)胞損傷。

【關(guān)鍵詞】 大黃素;肝細(xì)胞;冷缺血再灌注;細(xì)胞內(nèi)鈣離子濃度;凋亡

Effect of Emodin on intracellular calcium concentration ([Ca2+]i) andapoptosis of hepatic cells after simulated cold ischemia�瞨eperfusion

QI Xiang, L�a Yi, SHEN Nai�瞴ing, LIU Chang, LIU Xue�瞞in, WANG Bo

Department of Hepatobiliary Surgery, the First Affiliated Hospital, Medical School ofXi�餫n Jiaotong University, Xi�餫n 710061, China

ABSTRACT: Objective To investigate the effect of Emodin on intracellular calcium concentration ([Ca2+]i) and apoptosis of hepatic cells after simulated cold ischemia�瞨eperfusion. Methods Glucose�瞣xygen deprivation, low temperature, subsequent reoxygenation and rewarming were used to induce ischemia�瞨eperfusion injury model in cultured hepatic cells which were divided into 4 groups: control group and Emodin�瞭reated group(100, 10 and 1μmol/L groups). The intracellular calcium concentration ([Ca2+]i) and apoptosis rate were determined by flow cytometry (FCM) respectively; the content of lactate dehydrogenase (LDH) in supernatant was tested. Results Intracellular calcium fluorescence intensity in Emodin�瞭reated groups of high, medium and low density was 24.12±0.51, 26.35±1.34 and 39.12±1.94, respectively, which were significantly lower than 105.29±1.01 in control group(P<0.01). Apoptosis rate in Emodin�瞭reated groups of high, medium and low density was 0.0546±0.0041, 0.1064±0.0064 and 0.1190±0.0050, respectively, which were significantly lower than 0.2540±0.0141 in control group(P<0.01). LDH was (198.83±14.22)u/L and (179.67±18.57)u/L in Emodin�瞭reated groups of medium and high density respectively, which were significantly lower than (351.33±34.16)u/L in control group(P<0.01). Conclusion Emodin could reduce [Ca2+]i and inhibit apoptosis of hepatic cells after simulated cold ischemia�瞨eperfusion, thus protecting hepatic cells effectively醫(yī).學(xué)全.在.線網(wǎng)站f1411.cn.

KEY WORDS: Emodin; hepatic cell; ischemia�瞨eperfusion injury; intracellular free calcium concentration; apoptosis

大黃素[1��2]化學(xué)結(jié)構(gòu)為1，3，8�擦u基��6�布谆�蒽醌，具有抗炎、抗腫瘤、保護(hù)肝腎等多種生物學(xué)作用。有文獻(xiàn)報(bào)道[3��4]，大黃素可對(duì)細(xì)胞內(nèi)鈣離子濃度及細(xì)胞凋亡產(chǎn)生影響。鈣超載及凋亡均為缺血再灌注損傷機(jī)制之一。本課題組前期研究發(fā)現(xiàn)[5]，大黃素對(duì)肝細(xì)胞體外模擬冷缺血再灌注損傷具有保護(hù)作用。該保護(hù)作用是否與抑制肝細(xì)胞內(nèi)鈣超載及凋亡有關(guān)?大黃素對(duì)冷缺血再灌注后肝細(xì)胞內(nèi)鈣離子濃度及凋亡的影響未見(jiàn)相關(guān)報(bào)道。本研究擬通過(guò)觀察大黃素對(duì)模擬冷缺血再灌注后肝細(xì)胞內(nèi)鈣離子濃度及凋亡的影響，初步探討其對(duì)冷缺血再灌注損傷可能的保護(hù)機(jī)制，為其在臨床肝移植中的應(yīng)用提供一定的實(shí)驗(yàn)依據(jù)。

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