 |
 |
醫(yī)學論文范文:17β_雌二醇對新生牛血清誘導的血管平滑肌細胞增殖反應的影響及其機制
【摘要】 目的：探討雌甾_1,3,5(10)_三烯_3，17β_二醇(17β_雌二醇)對新生牛血清誘導血管平滑肌細胞(VSMCs)增殖反應的影響及其機制。方法：用新生牛血清刺激培養(yǎng)的VSMCs增殖，MTT法檢測17β_雌二醇對新生牛血清誘導的VSMCs增殖的影響，并用Western blot檢測caveolin_1蛋白，誘導型一氧化氮合酶(iNOS)及NO在此過程中的變化。結果：與對照組比，新生牛血清可促進VSMCs增殖(P<0.05)，17β_雌二醇作用24h后能明顯抑制上述作用(P<0.05);17β_雌二醇預處理可抑制新生牛血清誘導的VSMCs中caveolin_1，iNOS蛋白表達下降及NO釋放(P<0.05);17β_雌二醇受體阻斷劑1_{4_[2_(n,n_二甲氨基)乙氧基]苯｝_1,2_二苯_1_丁烯(他莫昔芬)預處理可部分逆轉17β_雌二醇的抑制作用(P<0.05)。結論：17β_雌二醇通過其受體可抑制新生牛血清誘導的VSMCs增殖，此過程與上調caveolin_1蛋白表達，激活iNOS，增加NO釋放有關。
【關鍵詞】 17β_雌二醇;caveolin_1;誘導型一氧化氮合酶;血管平滑肌細胞
Influence of 17β_estradiol on Vascular Smooth Muscle Cells Proliferation Induced
by Fetal Calf Serum and Its MechanismLIU Hai_mei1，ZHAO Xiao_feng2，XU Jin_wen3，LIN Gui_ping3，WANG Ting_huai3
(1 Department of Physiology，Guangzhou University of Chinese Medicine，Guangzhou 510006，China，2 Department of Physiology，Me_dical College of Shaoguan University，Shaoguan 512026，China，3 Department of Physiology，Zhongshan Medical College，Sun_Yat Sen University，Guangzhou 510080，China)
[Abstract]Objective： To investigate the influence of 17β_estradiol on vascular smooth muscle cells(VSMCs)proliferation induced by fetal calf serum and the related mechanism. Methods： The fetal calf serum was used to stimulate cultured VSMCs proliferation(P<0.05)，and the effects of estrogen on VSMCs proliferation were determined by MTT，and the expressions of caveolin_1 and inducible nitric oxide synthase(iNOS)were also detected by Western blot. Results： Compared with the normal control group， the fetal calf serum could increase the VSMCs proliferation(P<0.05)， and inhibit proliferation effect of fetal calf serum，pretreated with estradiol for 24 hours(P<0.05). Western bolt results further proved that 17β_estradiol inhibited the downregulation of caveolin_1， iNOS and NO induced by fetal calf serum， which was prevented by pretreatment with estrogen antagonist， Tamoxifen(P<0.05). Conclusion： 17β_estradiol can inhibit VSMCs proliferation induced by fetal calf serum， and this effect is mediated by estrogen receptor. 17β_estradiol increases caveolin_1 and iNOS protein expression， and NO release，leading to the inhibition of VSMCs proliferation induced by fetal calf serum醫(yī).學全.在.線網(wǎng)站f1411.cn.
[Key Words]17β_estradiol，caveolin_1，inducible nitric oxide synthase，vascular smooth muscle cell
血管損傷后，刺激因子誘導的血管平滑肌細胞(VSMCs)的過度增殖和遷移是再狹窄發(fā)生的關鍵[1_2]，臨床及流行病學資料顯示，雌甾_1,3,5(10)_三烯_3，17β_二醇(17β_雌二醇)具有心血管保護作用，我室前期的實驗研究也證實這點[3]，在離體培養(yǎng)的VSMCs中17β_雌二醇可抑制ET_1誘導的VSMCs增殖，此過程與抑制細胞外信號通路1/2(ERK1/2)有關。17β_雌二醇的血管保護作用與誘導NO生成有關，但在VSMCs中，17β_雌二醇通過何種途徑誘導NO合成，目前尚未明了。因此，本研究利用離體培養(yǎng)的兔胸主動脈VSMCs，觀察caveolin_1及誘導型一氧化氮合酶(iNOS)在17β_雌二醇誘導NO合成及抑制VSMCs增殖中的作用和機制。
