醫(yī)學(xué)免費(fèi)論文:細(xì)胞調(diào)控基因hCHK2多態(tài)性與食管癌易感性的研究

醫(yī)學(xué)免費(fèi)論文:細(xì)胞調(diào)控基因hCHK2多態(tài)性與食管癌易感性的研究

【關(guān)鍵詞】 細(xì)胞調(diào)控基因 hCHK2多態(tài)性 食管癌易感性

susceptibility of esophageal cancer ZHANG Hui�瞲ia, CHEN Yan, YIN Dong, et al

(School of Public Health, Xinjiang Medical University, Urumqi 830011, China)Abstract: Objective: To determine the association between hCHK2 polymorphism and the susceptibility of esophageal cancer in Han nationality. Methods: A case�瞔ontrol study was conducted. The polymorphism between hCHK2 gene at rs2278022、rs2602431 and rs2970077 position was analyzed by polymerase chain reaction�瞝igase detection reaction (PCR�睱DR) methods in 107 esophageal cancer cases and 204 non�瞔ancer controls. The frequencies of genotype and allele and the association between the genetic polymorphisms and cancer risk were analyzed. Results: No significant difference was observed in the frequency of hCHK2 gene at rs2278022、rs2602431 and rs2970077 position genotypes between cases and controls (P>0.05). Conclusion: The polymorphism of hCHK2 gene at rs2278022、rs2602431 and rs2970077 position is unlikely to be associated with the susceptibility of esophageal cancer醫(yī).學(xué).全.在.線f1411.cn.

Key words: hCHK2; esophageal cancer; susceptibility; genetic polymorphism隨著對(duì)細(xì)胞周期及其調(diào)控的深入認(rèn)識(shí)，學(xué)者們提出了“癌癥可能是一類細(xì)胞周期異常性疾病”的觀點(diǎn)[1]。絕大多數(shù)腫瘤細(xì)胞都存在若干細(xì)胞周期調(diào)控機(jī)制的改變，周期調(diào)控機(jī)制的異�？蓪�(dǎo)致細(xì)胞的持續(xù)性增殖，是腫瘤發(fā)生的生物學(xué)基礎(chǔ)。CHK2是近來(lái)新發(fā)現(xiàn)的一個(gè)細(xì)胞周期調(diào)控蛋白。在發(fā)生DNA損傷或復(fù)制阻滯后，細(xì)胞通過(guò)不同途徑激活CHK2，進(jìn)而作用于下游不同的靶蛋白，最終激活G1、S和(或)G2/M期檢測(cè)點(diǎn)機(jī)制，使細(xì)胞周期進(jìn)程發(fā)生阻滯，同時(shí)激活修復(fù)相關(guān)基因的轉(zhuǎn)錄，促進(jìn)細(xì)胞對(duì)損傷進(jìn)行修復(fù)。由于CHK2在細(xì)胞周期中具有重要的負(fù)調(diào)控作用，許多學(xué)者認(rèn)為CHK2起著抑癌基因的作用，它的異常會(huì)導(dǎo)致細(xì)胞周期檢測(cè)點(diǎn)對(duì)DNA損傷或復(fù)制阻滯不能發(fā)揮正常功能，從而引起腫瘤易感性增加，最終導(dǎo)致腫瘤的發(fā)生或發(fā)展。因此，我們采用聚合酶鏈?zhǔn)椒磻?yīng)�策B接酶檢測(cè)反應(yīng)(polymerase chain reaction�瞝igase detection reaction，PCR�睱DR)技術(shù)對(duì)新疆漢族人群107例食管癌患者和204例非食管癌患者進(jìn)行了hCHK2 rs2278022、rs2602431和rs2970077基因多態(tài)性的檢測(cè)，并分析探討了其基因多態(tài)性與食管癌遺傳易感性之間的關(guān)系，以期為食管癌的防治提供理論依據(jù)。

1材料與方法

1.1研究對(duì)象收集2005～2007年間新發(fā)的、由5所醫(yī)院(新疆醫(yī)科大學(xué)第一附屬醫(yī)院、第二附屬醫(yī)院、附屬腫瘤醫(yī)院、伊犁州新華醫(yī)院、伊犁州友誼醫(yī)院)經(jīng)內(nèi)窺鏡、X線及病理確診的漢族原發(fā)性食管癌患者107例作為病例組，其中男性76例，女性31例，并選擇與病例無(wú)血緣關(guān)系、同民族、同性別、年齡相差±5歲、同期住院的非食管癌患者204例作為對(duì)照組，其中男性151例，女性53例。所有研究對(duì)象采集外周靜脈血4 ml，EDTA抗凝，��20℃保存至提取DNA。全部調(diào)查對(duì)象均知情同意。

1.2方法

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