醫(yī)學(xué)免費(fèi)論文:Galectin3與肺腺癌淋巴及胸膜轉(zhuǎn)移的關(guān)系
【摘要】 目的 探討β半乳糖苷結(jié)合蛋白3(Galectin3)表達(dá)與肺腺癌淋巴及胸膜轉(zhuǎn)移的關(guān)系。方法 采用鼠抗人Galectin3單克隆抗體和免疫組化鏈霉素抗生物素蛋白-過(guò)氧化物酶(SP)法,檢測(cè)63例肺原發(fā)性腺癌和34例胸膜轉(zhuǎn)移性腺癌(均來(lái)自肺,影像學(xué)顯示為非直接侵襲病灶)中Galectin3的表達(dá)。結(jié)果 發(fā)現(xiàn)Galectin3同時(shí)存在于胞漿及胞核,未見(jiàn)單獨(dú)的胞核表達(dá)。肺原發(fā)性腺癌胞漿、胞核表達(dá)陽(yáng)性率分別為92.1%、31.7%,胞核表達(dá)與淋巴結(jié)轉(zhuǎn)移(P=0.03)、臨床分期有關(guān)(P<0.05)。胸膜轉(zhuǎn)移性腺癌胞漿、胞核陽(yáng)性率分別為97.1%、85.3%,胞核表達(dá)明顯高于肺原發(fā)性腺癌,差異有統(tǒng)計(jì)學(xué)意義(P<0.05)。結(jié)論 細(xì)胞核內(nèi)Galectin3可能增強(qiáng)肺腺癌細(xì)胞侵襲轉(zhuǎn)移潛能,可作為判斷淋巴結(jié)轉(zhuǎn)移和/或胸膜轉(zhuǎn)移的生物學(xué)指標(biāo)。
【關(guān)鍵詞】 β半乳糖苷酶類;癌,非小細(xì)胞肺;腫瘤轉(zhuǎn)移
pleural metastasis in lung adenocarcinoma醫(yī).學(xué)全.在.線網(wǎng)站f1411.cn
LONG Chengfeng, LIN Dianjie, CUI Jiyun, WAN Yunyan
(Department of Respiratoty Disease, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China)
To investigate the correlation between galectin3 and lymphatic metastasis and pleural metastasis in adenocarcinoma of the lung. Methods Immunohistochemistry was used to determine expression of galectin3 in 63 primary lung adenocarcinomas specimens and 34 adenocarcinomas metastatic of pleural specimens. Results The patterns of galectin3 expression were cytoplasmic and nuclear. All cases of nuclear galectin3 also showed a cytoplasmic immunoreactivity. In primary tumors the positive galectin3 expression was found in 92.1% of the cytoplasm and 31.7% of the nucleus. Only the nuclear location of galectin3 was associated with lymphatic metastasis (P=0.03) and clinical stages(TNM stages)(P<0.05). In pleural secondary adenocarcinomas the positive expression rate of galectin3 in the cytoplasm and nucleus was 97.1% and 85.3% respectively, and the nuclear staining was significantly higher than the primary tumour(P<0.05). Conclusion Nuclear expression of galectin 3 can enhance the invasive and metastatic potential of lung adenocarcinomas and can be used as a biological marker of lymph node and/or pleural metastasis.
Key words: Betagalactosidase; Carcinoma, nonsmall cell lung; Neoplasm metastasis 在肺癌的研究中發(fā)現(xiàn)β半乳糖苷結(jié)合蛋白3(Galectin3)多呈高表達(dá),但有關(guān)其與肺腺癌淋巴轉(zhuǎn)移及胸膜轉(zhuǎn)移的關(guān)系研究報(bào)道較少。本研究旨在應(yīng)用免疫組織化學(xué)SP染色方法來(lái)檢測(cè)肺原發(fā)性腺癌及胸膜轉(zhuǎn)移性腺癌中Galectin3的表達(dá),初步探討Galectin3與肺腺癌淋巴結(jié)轉(zhuǎn)移及胸膜轉(zhuǎn)移的關(guān)系。
1 資料與方法
1.1 資料
1.1.1 臨床資料 選取2006年至2007年山東省立醫(yī)院呼吸內(nèi)科因胸腔積液原因待查住院患者行內(nèi)科胸腔鏡活檢確診為胸膜轉(zhuǎn)移性腺癌的石蠟標(biāo)本34例,(來(lái)自肺,影像學(xué)顯示為非直接侵襲病灶),男15例,女19例,42~64歲,其中影像學(xué)或體格檢查發(fā)現(xiàn)伴淋巴結(jié)轉(zhuǎn)移(腫大)21例;另有2007年胸外科手術(shù)切除的肺腺癌石蠟標(biāo)本63例,男29例,女34例,45~74歲,其中伴有淋巴結(jié)轉(zhuǎn)移33例。所有病例取標(biāo)本前未行任何放化療,均有病例診斷結(jié)果。
1.1.2 試劑 實(shí)驗(yàn)所用Galectin3鼠抗人單克隆抗體、SP試劑盒(含二抗、三抗工作液)及DAB顯示劑均購(gòu)于福州邁新公司;蘇木素購(gòu)于北京中衫金橋生物公司。